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Invitrogen™ β-Gal Assay Kit
Provides reagents required to quickly measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing lacZgene
Brand: Invitrogen™ K145501
599.04 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer.
Description
- lacZis bacterial gene often used as reporter construct in eukaryotic transfection experiments
- Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
- β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG)
- Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer
- 10.7 gene expression
- Colorimetric detection
Beta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays
Specifications
| The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored. | |
| Colorimetric | |
| ONPG | |
| Beta-Galactosidase |
| β-Gal (lacZ) Assay | |
| Beta-Gal Assay Kit | |
| 80 mL |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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