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Invitrogen™ DNA Polymerase I, Large (Klenow) Fragment
DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities
Brand: Invitrogen™ 18012021
Includes: DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500mM Tris-HCl (pH 8.0), 100mM MgCl2, 500mM NaCl], vial of dilution buffer.
Description
Applications:
Fill-in of 5' overhangs . Synthesis of probes by random primers labeling method. Sequencing single- and double-stranded DNA. Site-directed mutagenesis.
Source:
Purified from E. coli expressing the Klenow fragment on a plasmid.
Performance and Quality Testing:
SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Unit Definition:
One unit incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.
Array CGH, Cloning, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Restriction Enzyme Cloning
Order Info
Shipping Condition: Approved for shipment on Wet or Dry Ice
Specifications
| DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact™ 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl2 , 500 mM NaCl], vial of dilution buffer. Store at -20°C. |
|
| DNA Polymerase I | |
| 100 Units | |
| No |
| DNA Polymerase | |
| 3' to 5' | |
| Approved for shipment on Wet or Dry Ice |
For Research Use Only. Not for use in diagnostic procedures.