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Invitrogen™ ChargeSwitch™ gDNA Mini Bacteria Kit
PCR, restriction enzyme digestion, cloning, Southern blotting, and sequencing downstream applications
Brand: Invitrogen™ CS11301
Includes:
- ChargeSwitch Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
- ChargeSwitch Resuspension Buffer (R4)
- ChargeSwitch Lysis Buffer (L14)
- ChargeSwitch Binding Buffer (B8)
- ChargeSwitch Wash Buffer (W12)
- ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
- Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
- RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
Description
The ChargeSwitch gDNA Mini Bacteria Kit uses magnetic bead-based technology to provide rapid and efficient purification of up to 12μg of genomic DNA from up to 0.5mL of Gram-positive or Gram-negative bacterial culture or an individual colony in less than 15 minutes after lysate preparation
- Optimized for higher-purity DNA extraction
- Improved performance with accompanying magnetic rack
- Cleaner purified plasmid for optimal downstream performance
- High quality, high yield plasmid DNA
- Compatible with MagnaRack™ magnetic rack
- Improved performance in downstream applications
- Less hazardous, less waste
DNA & RNA Purification & Analysis, DNA Extraction, Genomic DNA Purification, gDNA from Bacteria
Order Info
Shipping Condition: Room Temperature
Specifications
| All components of the ChargeSwitch™ gDNA (genomic DNA) Mini Bacteria Kit are shipped at room temperature. Upon receipt, store components as follows: Store Proteinase K at 4“C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: ChargeSwitch™ Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) ChargeSwitch™ Resuspension Buffer (R4) ChargeSwitch™ Lysis Buffer (L14) ChargeSwitch™ Binding Buffer (B8) ChargeSwitch™ Wash Buffer (W12) ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5) Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol) RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA) |
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| ChargeSwitch Chemistry | |
| Mini | |
| Fewer resources used and less waste, Less hazardous | |
| ChargeSwitch™ | |
| 50 Preps | |
| Genomic DNA |
| Kit | |
| Bacteria | |
| PCR, Southern Blotting, Sequencing, Cloning | |
| Not High-throughput Compatible (Manual) | |
| gDNA Mini Bacteria Kit | |
| Room Temperature | |
| 15 min |
For Research Use Only. Not for use in diagnostic procedures.