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Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)
Description
When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.
Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.
Order Info
Shipping Conditions: Dry Ice
Specifications
Specifications
| Concentration | 50 mM |
| Content And Storage | 5-(3-Aminoallyl)-dUTP, 50 mM (50 μL) Store at –70°C. |
| Form | Liquid |
| Label or Dye | Aminoallyl |
| Product Line | Ambion |
| Product Type | Nucleotides |
| Purification Method | HPLC |
| Quantity | 50 μL |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Nucleic Acid Labeling |
For Research Use Only. Not for use in diagnostic procedures.
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