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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set

Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal-to-noise ratios.

Brand: Invitrogen™ 88-8824-00

Product Code. 15284728

€ 204.00 / Each

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Alert:

Contains Sodium azide and formaldehyde

Includes:

IC Fixation Buffer
Permeabilization Buffer (10X)

This item is not returnable. View return policy

Description

In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation

Specifications

Product Line	eBioscience™
Product Type	Fixn/Perm Buffer Set
Quantity	1 kit
Solution Type	Fixation/Permeabilization Buffer

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Safety and Handling

Product Identifier

eBioscience Intracellular Fixation & Permeabilization Buffer Set

Signal Word

Danger

Hazard Category

Carcinogenicity Category 1A
Serious eye damage/eye irritation Category 1
Germ cell mutagenicity Category 2
Skin sensitiser Category 1
Specific target organ toxicity Category 2

Hazard Statement

H317-May cause an allergic skin reaction.
H318-Causes serious eye damage.
H341-Suspected of causing genetic defects.
H350-May cause cancer.
H371-May cause damage to organs.

Precautionary Statement

P201-Obtain special instructions before use.
P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P264-Wash hands thoroughly after handling.
P270-Do not eat, drink or smoke when using this product.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308+P313-IF exposed or concerned: Get medical advice/attention.
P310-Immediately call a POISON CENTER/doctor/.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Paraformaldehyde

Documents

Product Information

Manuals

Technical Data Sheet: Intracellular Fixation & Permeabilization Buffer Set Version: 14 March 2017 Rev. 10 File size: 124.2 kB

Scientific Resources

Posters

Poster: Expression of an immune checkpoint receptor VSIG4 defines new subsets of mouse peritoneal macrophages File size: 447.1 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2990891	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number88-8824-00
Lot Number2504455	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number88-8824-00
Lot Number2528101	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number88-8824-00
Lot Number2555740	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number88-8824-00
Lot Number2589190	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number88-8824-00

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