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Invitrogen™ IL-7 Mouse ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EMIL7X5
This item is not returnable.
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Description
The Mouse Interleukin-7 (Ms IL-7) ELISA quantitates Ms IL-7 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-7. Principle of the method The Mouse IL-7 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
Specifications
| P10168 | |
| 10 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Plasma, Serum, Supernatant | |
| 5 x 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 16196 | |
| <12% | |
| Assay Diluent Concentrate, Adhesive Plate Covers, SAV-HRP, Chromogen, Wash Buffer, Stop Solution, Biotinylated Detection Antibody, Pre-coated 96 well plates, Standard | |
| 5 x 96 Tests | |
| Bone Research | |
| 2°C to 8°C | |
| Mouse | |
| 4 hr. 45 min. |
| 8.23 pg/mL to 6000 pg/mL | |
| 10 pg/mL to 6000 pg/mL | |
| Biotin | |
| Cytokines & Receptors | |
| This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
| Mouse | |
| Colorimetric Microplate Reader | |
| A630026I06Rik,Il-7,hlb368 | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 50 μ;L; Serum, 50 μ;L; Supernatant, 100 μ;L | |
| A630026I06Rik, Il-7, hlb368 | |
| 1 hr. 20 min. |