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  5. Invitrogen™ Human IgA Uncoated ELISA Kit with Plates

Invitrogen™ Human IgA Uncoated ELISA Kit with Plates

Product Code. p-7091900
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Quantity:
20 x 96 Tests
Unit Size:
Pack of 20
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Product Code. 15570947

Brand: Invitrogen™ 885060076

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Description

ELISA

The Human Immunoglobulin A (IgA) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgA. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Human IgA, also known as human immunoglobulin A, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in mucosal secretions, such as saliva, tears, and breast milk, and plays a key role in defense against pathogens at mucosal surfaces. IgA works by binding to specific antigens and preventing them from entering the body's tissues. It can also activate complement and promote phagocytosis by immune cells. Human IgA can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious diseases.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01876, P01877
Assay Range 1.6-100 ng/mL
Assay Sensitivity 1.6 ng/mL
Content And Storage Capture Antibody Concentrate
Detection Antibody Concentrate
human IgA isotype control (standard), lyophilized, 50 ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Tetramethylbenzidine (TMB) Substrate Solution
96 Well Plate included with product Cat. Nos. ending in suffixes -22, -76, -86
Conjugate HRP
Immunoassay Kit Format Uncoated ELISA Kit with Plates
Product Type ELISA
Sample Type Plasma, Serum
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IgA1; IGHA1; IGHA2; Immunoglobulin A; Immunoglobulin Am1; Immunoglobulin Am2; immunoglobulin heavy constant alpha 1; Immunoglobulin Heavy Constant Alpha 2; immunoglobulin heavy constant alpha 2 (A2m marker)A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IgA1; IGHA1; IGHA2; Immunoglobulin A; Immunoglobulin Am1; Immunoglobulin Am2; immunoglobulin heavy constant alpha 1; Immunoglobulin Heavy Constant Alpha 2; immunoglobulin heavy constant alpha 2 (A2m marker);
Gene ID (Entrez) 3493, 3494
Gene Symbol IGHA1, IGHA2
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye HRP
Quantity 20 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL
Storage Requirements 2-8°C
Synonym Immunoglobulin A
Target Human IgA
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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