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Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Brand: Applied Biosystems™ 4389986
593.36 EUR valid until 2024-12-14
Use promo code "21632" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price
Description
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
- Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
- Formulated to ensure maximum sensitivity and reliability
- Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets
Includes:
- AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
- ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
- An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
- RNase inhibitor, to prevent degradation of RNA templates
- A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments
Order Info
Shipping Conditions: Wet Ice
Specifications
| Sufficient for 200 reactions at 50 μL reaction volume and includes: • 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening. • 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
|
| 1-step RT-qPCR | |
| Standard | |
| SYBR Green | |
| RNA-to-CT™, SYBR™ | |
| UP (Ultra Pure) | |
| Standard | |
| 200 Reactions | |
| 200 Reactions |
| SYBR | |
| AmpliTaq Gold DNA Polymerase | |
| 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System | |
| ROX (Pre-mixed) | |
| One-Step qRT-PCR Kit | |
| ArrayScript™ UP | |
| Wet Ice | |
| Gene Expression | |
| RNA |
For Research Use Only. Not for use in diagnostic procedures.