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Invitrogen™ Human GOLPH3 (aa 34-65) Control Fragment Recombinant Protein
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Quantity:
100 μL
Unit Size:
100µL
Description
Highest antigen sequence indentity to the following orthologs: Mouse (94%), Rat (94%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65982 (PA5-65982. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.
Specifications
Specifications
| Accession Number | Q9H4A6 |
| Concentration | ≥5.0 mg/mL |
| For Use With (Application) | Blocking Assay, Control |
| Formulation | 1 M urea, PBS with no preservative; pH 7.4 |
| Gene ID (Entrez) | 64083 |
| Name | Human GOLPH3 (aa 34-65) Control Fragment |
| Quantity | 100 μL |
| Regulatory Status | RUO |
| Gene Alias | 4733401N08Rik; 5730410D03Rik; AW413496; coat protein GPP34; coat-protein; Gm x 33; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi phosphoprotein 3 (coat-protein); golgi protein; golgi-associated protein; GOLPH3; GOPP1; GPP34; MIDAS; Mitochondrial DNA absence factor; trans-Golgi protein GM x 33; Vps74 |
| Common Name | GOLPH3 |
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