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Proteintech™ Human ENO2 ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous ENO2

Brand:  Proteintech™ KE00050-96T


Product Code. 16861466

  • € 269.00 / 96 tests

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Description

Description

  • Recognizes human ENO2
  • ENO2 (enolases) are cytoplasmic glycolytic enzymes that may be involved in differentiation
    • Three isoenzymes:
      • Alpha: expressed in most tissues
      • Beta: expressed in muscle tissue
      • Gamma (ENO2): isoform neuron-specific enolase (NSE), a homodimer is localized in neurons and neuroendocrine cells
        • Cancer diagnostic marker for brain tumors
        • Plays role in glycoloysis-related energy pathway
        • Might be involved in higher metabolic activity during day

Process:

  • Antibody specific for ENO2 pre-coated onto microwells
  • ENO2 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for ENO2 is added to detect captured ENO2 protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

NP_001966
0.11 ng/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
3.5 hr.
0.313 to 20 ng/mL
ISO 13485
Coated ELISA Kit
DAB
2026
Solid
<10%
-20°C to +4°C
P09104
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