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Proteintech™ Human ENO2 ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous ENO2
Brand: Proteintech™ KE00050-96T
Description
- Recognizes human ENO2
- ENO2 (enolases) are cytoplasmic glycolytic enzymes that may be involved in differentiation
- Three isoenzymes:
- Alpha: expressed in most tissues
- Beta: expressed in muscle tissue
- Gamma (ENO2): isoform neuron-specific enolase (NSE), a homodimer is localized in neurons and neuroendocrine cells
- Cancer diagnostic marker for brain tumors
- Plays role in glycoloysis-related energy pathway
- Might be involved in higher metabolic activity during day
- Three isoenzymes:
Process:
- Antibody specific for ENO2 pre-coated onto microwells
- ENO2 protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody specific for ENO2 is added to detect captured ENO2 protein
- For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
NP_001966 | |
0.11 ng/mL | |
Sandwich ELISA, Double Antibody | |
Plasma, Serum | |
Human | |
Human | |
<10% | |
Blue ice | |
3.5 hr. |
0.313 to 20 ng/mL | |
ISO 13485 | |
Coated ELISA Kit | |
DAB | |
2026 | |
Solid | |
<10% | |
-20°C to +4°C | |
P09104 |
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Safety and Handling
- 96TEST Human ENO2 ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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