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  5. Invitrogen™ Human APRIL ELISA Kit

Invitrogen™ Human APRIL ELISA Kit

Product Code. 12737676
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
Each
Pack of 10
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Product Code. 12737676

Brand: Invitrogen™ BMS2008

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527.44 EUR valid until 2025-12-31
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This item is not returnable. View return policy

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human APRIL
Biotin-Conjugate anti-human APRIL polyclonal antibody
Streptavidin-HRP
Human APRIL Standard lyophilized, 100ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

ELISA

The Human APRIL (Hu A PRoliferation-Inducing Ligand)) ELISA quantitates Hu APRIL in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APRIL. Principle of the method The Human APRIL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for A PRoliferation-Inducing Ligand), TALL2 (for TNF and ApoL-related Leukocyte-expressed Ligand 2), and TRDL 1a (for TNF related death ligand 1a) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O75888
Assay Range 0.78 to 50.0 ng/mL
Assay Sensitivity 0.4 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 8741
Gene Symbol APRIL,CD256,TALL-2,TALL2,TNLG7B,TRDL-1,UNQ383/PRO715,ZTNF2
Interassay CV 7.1%
Intraassay CV 8.1
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Synonym TNF 13, TALL 2, TRDL 1, CD256 antigen
Target APRIL
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 10 mins
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Safety and Handling

compliance-icons
Product Identifier
  • Human APRIL ELISA Kit
 Signal Word
  • Danger
 Hazard Category
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 1
  • Skin corrosion/irritation Category 1 B
 Hazard Statement
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P363-Wash contaminated clothing before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Phosphoric acid, 5-bromo-5-nitro-1,3-dioxane
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