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Proteintech™ Human APOB ELISA Kit

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous APOB

Brand:  Proteintech™ KE00158-96T


Product Code. 16851566

  • € 269.00 / 96 tests

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Description

Description

  • Recognizes human APOB
  • Apoloprotein B (APOB) is a plasma protein synthesizes primarily in the liver
    • Essential for assembly of VLDL in liver and intestine
    • Plays role in lipid and cholesterol metabolism
    • APOB gene encodes two isoproteins through mRNA editing (APOB-48 and APOB-100)
      • APOB-100: synthesizes by liver and essential for assembly of VLDL in liver
      • APOB-48: synthesizes by small intestine and essential for assembly of chylomicrons in intestine
    • Plasma APOB levels associated with coronary heart disease

Process:

  • Antibody specific for APOB pre-coated onto microwells
  • APOB protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for human APOB is added to detect captured human APOB protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications

Specifications

NM_000384
0.68 ng/mL
Sandwich ELISA, Double Antibody
Plasma, Serum
Human
Human
<10%
Blue ice
4.5 hr.
3.9 to 250 ng/mL
ISO 13485
Coated ELISA Kit
DAB
338
Solid
<10%
-20°C to +4°C
P04114
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