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Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Brand: Thermo Scientific™ 88224

178.00 EUR valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 12393730

€ 267.00 / Pack of 25

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price

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Description

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin
Versatile – purify proteins using native or denaturing conditions
Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges
Cost-effective – reuse the same batch of resin at least five times
Easy to use – pre-formulated buffers available for kit formats

Available Formats:

Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
Purification Kits – complete kits in all three column sizes
Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns

Specifications

Capacity (Metric)	0.2 mL
Content And Storage	Store at 4°C
Product Line	HisPur™
Target	His-Tagged Protein
Stationary Phase	Divalent Nickel, Metal Chelate
Column Type	Affinity, Agarose Resin

Description	HisPur Ni-NTA Spin Columns, 0.2mL
Form	Liquid Suspension
Quantity	25 Columns
Type	Spin Column
Format	Microcentrifuge, Spin Column

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Safety and Handling

Product Identifier

HisPur Ni-NTA Spin Columns, 0.2 mL

Signal Word

Danger

Hazard Category

LONG-TERM AQUATIC HAZARD Chronic 3
Carcinogenicity Category 1A
Reproductive toxicity Category 1B
Respiratory sensitiser Category 1
Skin sensitiser Category 1
Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

H317-May cause an allergic skin reaction.
H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H350-May cause cancer.
H360D-May damage the unborn child.
H373-May cause damage to organs.
H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

P260-Do not breathe dust/fume/gas/mist/vapours/spray.
P273-Avoid release to the environment.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
P405-Store locked up.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Sulfuric acid, nickel(2+) salt, hydrate

Documents

Product Information

Manuals

User Guide: HisPur Ni-NTA Spin Columns Version: C File size: 131.3 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberSPECS	Certificate TypeCertificate of Analysis	Date15/10/2024	Catalog Number88224
Lot NumberZG395683	Certificate TypeCertificate of Analysis	Date17/08/2024	Catalog Number88224
Lot NumberZE386959	Certificate TypeCertificate of Analysis	Date22/05/2024	Catalog Number88224
Lot NumberZA381388	Certificate TypeCertificate of Analysis	Date25/01/2024	Catalog Number88224
Lot NumberYI370096	Certificate TypeCertificate of Analysis	Date29/09/2023	Catalog Number88224

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Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL > Spin columns, 0.8mL-capacity, 0.2mL resin; 25 columns

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