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Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.
Brand: Thermo Scientific™ 88831
144.57 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Product Code. 13464289
This item is not returnable.
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Description
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.
Additional Information and Online Ordering
The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs.Highlights:
High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification
Fast – protocol is completed in 1 hour
Scalable – process microliter to milliliter sample volumes
Versatile – purify proteins using native or denaturing conditions
Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives
Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)
Specifications
| Store at 4°C | |
| HisPur™ | |
| Recombinant | |
| Magnetic Affinity Bead |
| Liquid | |
| 2 mL | |
| His | |
| Nickel-NTA |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Ni-NTA Magnetic Beads
Signal Word
- Warning
Hazard Category
- Skin sensitiser Category 1
Hazard Statement
- H317-May cause an allergic skin reaction.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : sulphuric acid
For Research Use Only. Not for use in diagnostic procedures.