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Thermo Scientific™ HisPur™ Cobalt Resin
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
Brand: Thermo Scientific™ 89964
87.23 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.
Additional Information and Online Ordering
HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co2+)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni2+chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.Highlights:
High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Low metal leaching – no metal contamination in eluted histidine-tagged protein sample
Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives
Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates
Cost effective – resin can be reused several times or discarded after single use
Superior – performs better than other commercially available IMAC Resins
Available Formats:
- Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
- Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
- Purification Kits – complete kits in all three column sizes
- Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
- 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
- Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin
Includes:
Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer
Specifications
| HisPur Cobalt Resin | |
| Liquid Suspension | |
| 10 mL | |
| His-Tagged Protein | |
| Metal Chelate, Divalent Cobalt | |
| Affinity, Agarose Resin |
| Store at 4°C | |
| HisPur™ | |
| Recombinant | |
| Resin | |
| Bottle |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HisPur Cobalt Resin
Signal Word
- Danger
Hazard Category
- Flammable liquid Category 3
- Carcinogenicity Category 1B
- Reproductive toxicity Category 1B
- LONG-TERM AQUATIC HAZARD Chronic 2
Hazard Statement
- H226-Flammable liquid and vapour.
- H350-May cause cancer.
- H360F-May damage fertility.
- H411-Toxic to aquatic life with long lasting effects.
- EUH208-Contains . May produce an allergic reaction.
Precautionary Statement
- P201-Obtain special instructions before use.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P273-Avoid release to the environment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : Cobalt chloride(CoCl2), hexahydrate
For Research Use Only. Not for use in diagnostic procedures.