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  5. Invitrogen™ Mouse HGF ELISA Kit

Invitrogen™ Mouse HGF ELISA Kit

Codice prodotto. 17105739
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Dimensione della confezione:
1 set
Each
Pack of 10
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Codice prodotto. 17105739

Marca: Invitrogen™ EMHGFX5

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Les retours ne sont pas autorisés pour ce produit. Consulta la politica di reso

Descrizione

ELISA

The Mouse Hepatocyte Growth Factor(Ms HGF) ELISA quantitates Ms HGF in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms HGF. Principle of the method The Mouse HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
TRUSTED_SUSTAINABILITY

Specifica

Accession Number Q08048
Assay Range 0.614 to 60 ng/mL
Assay Sensitivity 400 pg/mL
Calibration Range 400 pg/mL to 60 ng/mL
Description HGF ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Chemokines and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of HGF in Samples
Detectable Analytes HGF
Assay Solid-phase sandwich ELISA
Conjugate HRP
Detection Method Colorimetric
Product Type ELISA
Protein Family Chemokine & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Sufficient For 5 x 96 assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 15234
Gene Symbol C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF
Host Species Mouse
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Apoptosis
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF
Target HGF
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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