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Gibco™ DMEM, high glucose, no glutamine, no phenol red
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
€ 25.86
Specifications
| Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|---|---|
| Cell Line | HeLa, 293, Cos-7, and PC-12 |
| Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
| Classification | Animal Origin-free |
| Concentration | 1 X |
Requires: Supplementation
Description
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: High Glucose
Without: L-glutamine, Phenol Red, Sodium Pyruvate, HEPES
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Compliance
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| Sterile-filtered | |
| No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| Room Temperature |
| HeLa, 293, Cos-7, and PC-12 | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| High Glucose | |
| Sustainable packaging | |
| Gibco™ |
Safety and Handling
missing translation for 'shelfLife' : 12 Months From Date of Manufacture
In vitro diagnostic