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  8. 11540446

Gibco™ Advanced DMEM/F-12
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Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Brand:  Gibco™ 12634010

21.95 EUR valid until 2025-03-29
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Product Code. 11540446

  • € 32.93 / 500mL

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Requires : Supplementation
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Description

Description

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:
With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine, HEPES

The complete formulation is available.

Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

MRC-5, SP2, Vero, WI-38 and Jurkat
1 X
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterile-filtered
High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
cGMP-compliant under the ISO 13485 standard
500 mL
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Animal Origin
Liquid
Reduced Serum
Sterile-filtered
No Glutamine, No HEPES
Gibco™
Room Temperature
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Safety and Handling

Product Identifier
  • Methyl benzoate

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4

Hazard Statement
  • H302-Harmful if swallowed.

Precautionary Statement
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.

Supplemental information
  • MIXTURE LIST-Contains : Methyl benzoate

SDS
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2715617 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number12634010
Lot Number2660099 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number12634010
Lot Number2660102 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number12634010
Lot Number2276712 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number12634010
Lot Number2660106 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number12634010
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Gibco™ Advanced DMEM/F-12 > Quantity: 500 mL

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