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Thermo Scientific™ GeneBLAzer™ RAR-beta DA Assay Kit

GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

Brand:  Thermo Scientific™ K1407

Product Code. 10480164

  • € 1204.00 / Each
Estimated Shipment: 16-01-2025
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Description

Description

GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Cell-based beta lactamase reporter gene
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
• RAR beta HEK 293T DA cells (K1407A)
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Fluorescence
RARβ
9-cis-retinoic acid
BLA (Beta-Lactamase)
RAR beta, NR1B2, RARB
293T (HEK)
Nuclear Receptors
none
Division-Arrested Cells
GeneBLAzer™ RAR-beta DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial Quantity of Cells, 293T (HEK) Cell Line, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
Pharma and biopharma, target and lead identification and validation, target-based adme/tox assays, Drug discovery and development, nuclear receptor biology, cell-based nuclear receptor reporter assays, target-based Adme/tox assays, cellular adme assays, nuclear receptor cellular adme assays
RAR beta HEK 293T DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg
1 plate
293T (HEK)
Division-Arrested Cells Assay Kit
GeneBLAzer™
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