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Thermo Scientific™ GeneBLAzer™ NTSR1 CHO-K1 DA Assay Kit

Product Code. 10583064
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Quantity:
1 Kit
Unit Size:
Each
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Product Code. 10583064

Brand: Thermo Scientific™ K1773

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Description

The GeneBLAzer™ NTSR1 CHO-K1 DA cells contain the human Neurotensin Receptor 1 (NTSR1) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line.

The GeneBLAzer™ NTSR1 CHO-K1 DA cells contain the human Neurotensin Receptor 1 (NTSR1) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase reporter gene under control of the NFAT response element.

The GeneBLAzer™ NTSR1 CHO-K1 DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, GeneBLAzer™ NTSR1 CHO-K1 DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_002531
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ NTSR1 CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ NTSR1 CHO-K1 DA Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ NTSR1 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) GPCRs
Gene Symbol NTSR1
Population Status Pool of Stable Cells
Product Line GeneBLAzer
Protein Family GPCR (G-Protein Coupled Receptors)
Quantity 1 Kit
Readout End Point
Reporter Gene BLA (Beta-Lactamase)
Target Entry NTSR1
Type Division-Arrested Cells Assay Kit
Cell Line U2OS
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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