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Thermo Scientific™ GeneBLAzer™ M4-Gqo5-NFAT-bla CHO-K1 Cells

Product Code. 10703364
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Quantity:
2 x 106 Cells
Unit Size:
Each
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Product Code. 10703364

Brand: Thermo Scientific™ K1741

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Description

GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 4 receptor (M4) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line.

GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 4 receptor (M4) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cells (Cat. No. K1536) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Chimeric G protein, Gqo5.

The GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Cabachol. In addition, GeneBLAzer™ M4 Gqo5-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The clinical implications of the M4 receptor are unknown, however studies with knockout mice suggest that M4 may have implications in dopaminergic function, karatinocyte migration and wound healing, anxiolysis, and analgesia. In addition, M4 may be important in memory circuits and have implications in Alzheimer's Disease. Additional information on the muscarinic receptors can be found in literature.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000741
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ M4 Gqo5 CHO-K1 DA cells
• GeneBLAzer™ M4 Gqo5 CHO-K1 DA Assay Protocol
• Certificate of Analysis
• Solution D
• LiveBLAzer™-FRET B/G Loading Protocol

The GeneBLAzer™ M4-Gqo5-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Description Population Status: Single Cell Clone Origin
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol M4
Includes GeneBLAzer™ M4 Gqo5 CHO-K1 DA cells, GeneBLAzer™ M4 Gqo5 CHO-K1 DA Assay Protocol, Certificate of Analysis, Solution D, LiveBLAzer™-FRET B⁄G Loading Protocol
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry M4
Type Dividing Cells
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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