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Thermo Scientific™ GeneBLAzer™ GRPR CHO-K1 DA Assay Kit

The GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells contain the human Gastrin Releasing Peptide Receptor (GRPR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line.

Brand: Thermo Scientific™ K1637

Product Code. 10676214

€ 1008.00 / Each

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Description

The GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells contain the human Gastrin Releasing Peptide Receptor (GRPR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line. GeneBLAzer™ NFAT-bla CHO-K1 (Cat. No. K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.

The GRPR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, GRPR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

Accession Number	NM_005314.2
Cell State	Division-Arrested Cells
Detection Method	Fluorescence
Gene Symbol	GRPR
Product Line	GeneBLAzer™, T-REx™
Reporter Gene	BLA (Beta-Lactamase)
Type	Division-Arrested Cells Assay Kit
System Type	GeneBLAzer™

Assay Entry	Cell-based beta lactamase reporter gene
Content And Storage	Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ GRPR CHO-K1 DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ GRPR CHO-K1 DA Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ GRPR CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application)	GPCRs
Population Status	Single Cell Clone Origin
Quantity	8 x 10⁶ Cells
Target Entry	GRPR
Cell Line	CHO-K1
Druggable Target	GPCR

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Thermo Scientific™ GeneBLAzer™ GRPR CHO-K1 DA Assay Kit

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