missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ pCMV-Gaussia-Dura Luc Vector for Luciferase Assays
Drive mutated Gaussia luciferase expression with vectors containing a multiple cloning site (MCS) or control promoter (CMV or TK) for extended signal output.
Brand: Thermo Scientific™ 16191
82.00 EUR valid until 2025-03-29
Use promo code "24111" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Description
Thermo Scientific™ pMCSGaussia-Dura Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for glow-analysis of gene regulation using the naturally secreting Gaussia luciferase reporter.
The Gaussia Dura Luc Vectors contains a mutant form of the Gaussia luciferase gene that confers better bioluminescent signal stability than native luciferase. Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.Highlights:
- Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
- Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
- Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
- Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
- High-copy pUC bacterial DNA replication origin
- Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization
Specifications
| pCMV-Gaussia-Dura Luc Vector | |
| Restriction Enzyme/MCS | |
| 10 μg | |
| Gaussia Dura Luciferase | |
| Reporter Assays |
| Luc Vector | |
| Transfection | |
| Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C. | |
| Puromycin | |
| CMV |
Product Suggestions
Customers who viewed this item also viewed
Viewing 1-5 of 9
-
Catalog No. 12559069
183,00 € / Pack of 10 -
Catalog No. 15955590
263,00 € / 30 reactions -
Catalog No. 10017003
114,00 € / 100mL -
Catalog No. 10729174
311,00 € / 500µL -
Catalog No. 15137105
9.870,00 € / Each -
Catalog No. 13444259
190,00 € / 50mL -
Catalog No. 11514270
862,00 € / 100µL -
Catalog No. 12152240
774,00 € / Each -
Catalog No. 12381853
123,00 € / 10µg
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Thermo Scientific™ pCMV-Gaussia-Dura Luc Vector for Luciferase Assays > pCMV-Gaussia-Dura Luc Vector
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction