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Thermo Scientific™ HpyF3I (DdeI) (10 U/μL)
The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).
Specifications
| Concentration | 10 U/μL |
|---|---|
| Methylation Sensitivity | Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
| Enzyme | HpyF3I (DdeI) |
| Compatible Buffer | 10X Buffer Tango |
| Sensitive to Heat Inactivation | Yes |
Description
Lambda DNA digested with HpyF3I (DdeI), 1.4% agarose, 104 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Highlights
- Superior quality -stringent quality control and industry leading manufacturing process
- Convenient color-coded Five Buffer System
- Includes universal Tango buffer for double-digestions
- BSA premixed in reaction buffers
- Wide selection of restriction endonuclease specificities
Specifications
| 10 U/μL | |
| HpyF3I (DdeI) | |
| Yes | |
| No | |
| Traditional Cloning |
| Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | |
| 10X Buffer Tango | |
| 37°C | |
| Restriction Enzyme |
For Research Use Only. Not for use in diagnostic procedures.