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Thermo Scientific™ GeneJET Plasmid Miniprep Kit

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.

Brand: Thermo Scientific™ K0503

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Product Code. 10242490

€ 371.00 / 250 reactions

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Description

GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights
• Efficient—high yields of up to 20 μg of high quality plasmid DNA
• Fast—procedure takes less than 14 minutes
• Convenient—no phenol/chloroform extraction or alcohol precipitation required
• Pure—purified DNA is immediately ready to use

Applications
• Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:
• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation

Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. Components Resuspension Solution Lysis Solution Neutralization Solution Wash Solution (concentrated) RNase A Solution Elution Buffer Spin Columns Collection Tubes Detailed Protocol

Specifications

Column Type	Spin Column
Isolation Technology	Silica Column
Sample Type	Bacteria
High-throughput Compatibility	Compatible (Vacuum manifold)
Product Line	GeneJET
Quantity	250 Preps
Test Time	<14 min

Format	Spin Column
Scale	Mini
For Use With (Application)	PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
Prep Scale	< 100 μg (Small-Scale) Plasmid DNA
Product Type	Plasmid MiniPrep Kit
Target	Plasmid DNA

Product Suggestions Safety and Handling

Safety and Handling

Signal Word

Warning

Hazard Category

Acute toxicity Category 4
Serious eye damage/eye irritation Category 2
Skin corrosion/irritation Category 2

Hazard Statement

H302-Harmful if swallowed.
H315-Causes skin irritation.
H319-Causes serious eye irritation.

Precautionary Statement

P264-Wash hands thoroughly after handling.
P270-Do not eat, drink or smoke when using this product.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P330-Rinse mouth.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Sodium lauryl sulfate, Sodium hydroxide

Documents

Product Information

Manuals

User Guide: GeneJET Plasmid Miniprep Kit, K0502 Version: Jan. 2015 File size: 145.6 kB

User Guide: GeneJET Plasmid Miniprep Kit Version: Jan. 2015 File size: 55.8 kB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2811034	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberK0503
Lot Number2810539	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberK0503
Lot Number2820488	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberK0503
Lot Number2814372	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberK0503
Lot Number2789240	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberK0503

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