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Thermo Scientific™ EZ-Link™ Maleimide Protein Labeling Kit
A convenient method to label proteins with varying amounts of maleimide for crosslinking or conjugation with a thiol/sulfhydryl containing moiety. Sulfo-SMCC reacts with primary amines on a protein to form a covalently attached maleimide group that can be further reacted with a thiol/free cystine.
Brand: Thermo Scientific™ C20045
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Description
- A convenient method to label proteins with varying amounts maleimide for crosslinking or conjugation with a thiol/sulfhydryl containing moiety
- Sulfo-SMCC contained in this kit readily reacts with the primary amines on a protein to form a covalently attached maleimide group which can be further reacted with a reactive thiol or free cystine to create diverse bioconjugates
- Thermo Scientific™ Sulfo-SMCC is a heterobifunctional crosslinker which contains a sulfonated N-hydroxysuccinimide (NHS) ester and maleimide group that allow covalent conjugation of amine- and thiol/sulfhydryl-containing molecules
- Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins
- This kit contains sufficient reagents for five labeling reactions of up to 100 μL sample with a concentration of 1-10 mg/mL
- Sulfo-SMCC reagent readily dissolves in water
Specifications
| 1 kit | |
| Amine-to-sulfhydryl | |
| Wet Ice | |
| EZ-Link™ |
| Contains 5 vials of sulfo-SMCC, 5 Zeba Dye & Biotin Removal columns, and BupH™ Phosphate Buffered Saline pack. Store sulfo-SMCC desiccated at –20°C, Zeba Dye and Biotin Removal columns at 2–8°C. |
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| Chemical Labeling | |
| Sulfo-NHS, Maleimide |