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Thermo Scientific™ CellSensor™ NFAT HEK 293T DA Assay Kit
The CellSensor™ NFAT HEK 293T DA (Division Arrested) cells contain a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells.
Brand: Thermo Scientific™ K1455
This item is not returnable.
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Description
The CellSensor™ NFAT HEK 293T DA (Division Arrested) cells contain a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at a fixed concentration of PMA. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background or these cells can be used in other assays where a sensitive readout to intracellular changes in calcium are needed.
Specifications
| Division-Arrested Cells | |
| GPCRs, Signaling Pathway | |
| CellSensor™ | |
| Division-Arrested Cells Assay Kit | |
| Signaling Pathway |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes: • CellSensor™ NFAT HEK293T DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • CellSensor™ HEK293T CHO-K1 Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The CellSensor™ NFAT HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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| CellSensor™ NFAT HEK293T DA cells, LiveBLAzer™-FRET B/G Loading Kit 70 μg, Solution D, CellSensor™ HEK293T CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis | |
| 1 plate | |
| CellSensor™ |
For Research Use Only. Not for use in diagnostic procedures.