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Corning™ DMEM (Dulbecco's Modified Eagle's Medium)
Corning™ DMEM (Dulbecco's Modified Eagle's Medium) support cell culture growth and substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over original formulation. Products undergo a variety of tests to determine functionality and toxicity using growth-promotion analysis.
Brand: Corning™ 15-013-LB
This item is not returnable.
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Description
- Modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME
- Original formulation was used to culture embryonic mouse cells
- Has been modified to support variety of normal and transformed cells, such as primary cultures of mouse and chicken cells
- Formulations can differ in amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine
- Tested to determine pH and osmolality
- Low levels of endotoxin, and absence of contamination
- Tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Chromogenic Assay
- Osmolality: 335±mOsm/kg H2O
- Mycoplasma: Tested Negative
- Sterility: Pass
Specifications
| 2 to 8°C | |
| Liquid | |
| Glucose, Sodium Pyruvate | |
| <0.25EU/mL | |
| To assess growth promotion and cytotoxicity characteristics |
| 1 X | |
| DMEM Media | |
| No Glutamine | |
| Inorganic Salts: 200mg/L CaCl2 (anhydrous), 0.1mg/L Fe(NO3)3.9H2O, 400mg/L KCl, 97.7mg/L MgSO4 (anhydrous), 6400mg/L NaCl, 125mg/L NaH2PO4.H2O, 3700mg/L NaHCO3; Amino Acids: 84mg/L L-Arginine. HCl, 62.57mg/L L-Cystine.2HCl, 30mg/L Glycine, 42mg/L L-Histidine.HCl.H2O, 104.8mg/L L-Isoleucine, 104.8mg/L L-Leucine, 146.2mg/L L-Lysine. HCl, 30mg/L L-Methionine, 66mg/L L-Phenylalanine, 42mg/L L-Serine, 95.2mg/L L-Threonine, 16mg/L L-Tryptophan, 103.79mg/L L-Tyrosine.2Na.2H2O, 20mg/L L-Valine; Vitamins: 4mg/L D-Calcium pantothenate, 4mg/L Choline chloride, 4mg/L Folic Acid, 7.2mg/L i-Inositol, 4mg/L Nicotinamide, 4mg/L Pyridoxine. HCl, 0.4mg/L Riboflavin, 4mg/L Thiamine. HCl; Other: 4500mg/L D-Glucose, 15mg/L Phenol red. Na, 110mg/L Sodium pyruvate; Added: 584mg/L L-glutamine Powder, 20 mL/L 200 mM Solution. | |
| 1 x 20 L |