missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Competent Cells
  5. 16440054

Thermo Scientific™ BL21(DE3) Competent Cells

Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

Brand:  Thermo Scientific™ EC0114

100.01 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 16440054

  • € 137.00 / 20 reactions

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

  • Suitable for the expression of non-toxic heterologous genes
  • The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG
  • BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.
  • High transformation efficiency: >1x107 cfu/μg pUC19 DNA
  • Specifically constructed for high-level expression of recombinant proteins
  • Genetic markers that minimize RNA and protein degradation
  • Convenient two reactions per vial packaging
  • S.O.C. Outgrowth medium included
  • Applications:  BL21(DE3) Competent Cells are suitable for high-level T7 expression of recombinant proteins
  • Genotype: F– ompT hsdSB (rB–, mB–) gal dcm (DE3)

Note: Not suitable for maintenance of expression plasmids. Use DH10B or DH5α competent cells that do not carry the gene for T7 RNA polymerase for plasmid DNA propagation (Cat. Nos. EC0112, EC0113).

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Chemically Competent
Dry Ice (-80°C)
E. coli
Cloning
Lacks F' Episome
BL21(DE3)
Tube
20 Reactions (10 x 100 μL)
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.