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Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer
The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.
Brand: Applied Biosystems™ 4398848
Includes: AmpliTaq 360 Buffer kit is supplied 1.5mL of each 10X AmpliTaq™ 360 Buffer, 25mM M2Cl2, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl2, and 360 GC Enhancer is sufficient for use with 250U of enzyme.. All solutions are prepared in deionized, filter-sterilized water.
Description
The Applied Biosystems AmpliTaq 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA Polymerase for situations when the reagents supplied with the enzyme are not sufficient for the experimental needs. When used with or without the optional 360 GC Enhancer, this buffer amplifies a vast range of DNA sequence contexts. AmpliTaq 360 DNA Polymerase delivers 360° coverage for a full range of targets.
The AmpliTaq 360 Buffer Kit is designed for use with challenging targets including AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can be reproducibly amplified with a single reagent under standardized conditions. Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 Polymerase as a superior-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets.
Features
- Optimized for the broad range of targets
- Robust amplification of GC-rich sequences with 360 GC Enhancer
- Achieves high quality sequencing data
Notes
- GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.
- See user's manual or package insert for limited label license and trademark information.
Order Info
Approved for shipment at Room Temperature or on Wet Ice
Specifications
| • 10X AmpliTaq 360 Buffer, 1.5 mL • 25 mM MgCl2, 1.5 mL • 360 GC Enhancer, 1.5 mL Store at -15°C to -25°C. Product is guaranteed up to 12 months as indicated by the expiration date. |
|
| AmpliTaq 360 DNA Polymerase | |
| Sustainable packaging | |
| 1.5 mL | |
| Standard PCR | |
| Kit |
| High | |
| Standard | |
| GC Enhancer | |
| Room Temp or Wet Ice | |
| 5 U/μL | |
| 1.5 mL |
For Research Use Only. Not for use in diagnostic procedures.