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Invitrogen™ MEGAclear™ Transcription Clean-Up Kit
Quickly cleanup large-scale transcription reactions
Brand: Invitrogen™ AM1908
This item is not returnable.
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Description
MEGAclear Kit is designed for quick cleanup of any large-scale transcription reaction. MEGAclear Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.- Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
High recovery from 1ng to 500μg of input
Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
Quick protocol—purify RNA in less than 30 minutes - Using the MEGAclear Kit: Protocol is quick and easy. Transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
Specifications
| Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature. | |
| Tube | |
| mRNA, Synthetic mRNA | |
| In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products | |
| Not High-throughput Compatible (Manual) | |
| Ambion™, MEGAclear™ | |
| 20 Preps | |
| Room Temperature | |
| 500 μg |
| Purified Synthetic mRNA, Purified mRNA | |
| Silica Spin Column | |
| mRNA, Synthetic mRNA | |
| Vacuum Manifold, Centrifuge | |
| 20 Preps | |
| mRNA purification kit, Purification kit | |
| 30 min | |
| 1 ng to 500 μg of dsRNA>200b or ssRA>100bp |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- MEGAclear Transcription Clean-Up Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Skin corrosion/irritation Category 1 C
Hazard Statement
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2-Mercaptoethanol
For Research Use Only. Not for use in diagnostic procedures.