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Invitrogen™ ChargeSwitch™ Total RNA Cell Kit
Total RNA purification using an aqueous-based method
Brand: Invitrogen™ CS14010
Includes: ChargeSwitch Magnetic Beads, Proteinase K, DNase I, DNase I Buffer, Lysis Buffer, Binding Buffer, Wash Buffers, and Elution Buffer
Description
The ChargeSwitch™ Total RNA Cell Kit is ideal for the purification of up to 25μg of total RNA from up to 1 x 106 cultured mammalian cells or from 1mL overnight bacterial culture, including full-length RNA and small molecular weight RNA species. This kit offers a unique aqueous-based method for purifying total RNA from cultured mammalian or bacterial cells resulting in exceptionally clean and pure RNA with high integrity. ChargeSwitch technology eliminates the use of common enzymatic inhibitors such as phenol, chaotropic salts or ethanol, resulting in exceptionally clean RNA. Due to its unique format, this kit also performs exceptionally well at concentrations useful for greater sensitivity in downstream analyses, yielding faster scientific results. The ChargeSwitch technology format is scalable and is a highly-sensitive RNA extraction method for successful downstream applications, including gene expression studies, promoter studies, siRNA, gene regulation, transcriptional analysis, RT-PCR, and qRT-PCR. This unique format also allows flexibility for application in high-throughput automation.
- Effectively captures up to 25μg of total RNA from up to 1 x 106 cultured mammalian cells or 1mL bacterial culture
- Purifies a wide range of transcripts including increasingly important small RNAs (smaller than 200 nucleotides)
- Provides the ability to purify greater yields of RNA from very small sample sizes
- Eliminates use of common enzymatic inhibitors providing exceptionally clean RNA
- Results in improved sensitivity in downstream analyses
- Allows flexibility for high-throughput applications
- Automated protocols, high-throughput compatible
Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge attracting RNA. At higher pH, the surface chemistry is neutral, thereby releasing any attraction to bound RNA. 50 preps.
Automated Nucleic Acid Purification, Automated Total RNA Isolation, DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues, Total RNA from Bacterial Samples
Specifications
| The ChargeSwitch™ Total RNA Cell Kit includes ChargeSwitch™ Magnetic Beads, Proteinase K, DNase I, DNase I Buffer, Lysis Buffer, Binding Buffer, Wash Buffers, and Elution Buffer. Store Proteinase K and Lysis Buffer at 4°C, DNase I at -20°C, and all remaining components at room temperature. All components are guaranteed stable for 6 months when stored properly. | |
| 15 min. | |
| Total RNA | |
| Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction | |
| 50 preps | |
| Up to 10^6 cells | |
| ChargeSwitch™ | |
| Magnetic Bead |
| ChargeSwitch Chemistry | |
| Cells, Bacteria | |
| High-throughput Compatible, Automated Protocols | |
| ChargeSwitch™ | |
| Mini | |
| Total RNA | |
| 40 μg |
For Research Use Only. Not for use in diagnostic procedures.