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Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | GMCSF |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 2 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 2-250 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P10145 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards.,96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3576 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Interleukin-8, CXCL8, NAP-I |
Target Species | Human |
---|---|
Conjugate | Biotin |
Gene Symbol | CANDF6,IL-17F,ML-1,ML1 |
Total Assay Time | 24 hr. 30 min. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 4 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 4-500 pg/mL |
For Use With (Application) | ELISA |
Test Time | 1 hr. 45 min. |
Accession Number | P01375 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Capture Antibody: Pre-titrated; purified antibody,Detection Antibody: Pre-titrated; biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP Concentrate (100X),Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86) |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 7124 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Synonym | Tumor Necrosis Factor alpha, tnf-a, tnf-alpha |
Invitrogen™ IL-1 beta Human ELISA Kit
Quantifies natural and recombinant human IL-1β in serum, buffered solution, or cell culture medium
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 6.6% |
Intraassay CV | 4.4% |
Gene Symbol | IL-1,IL1-BETA,IL1F2 |
Total Assay Time | 2 hr. 55 min. |
Sample Volume | Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | 1 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 3.9-250 pg/mL |
For Use With (Application) | Colorimetric |
Test Time | 1 hr. 20 min. |
Accession Number | P01584 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | 1pg/mL |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3553 |
Sample Type | Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ TNF alpha Human ELISA Kit
Each ready-to-use kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 7.5% |
Intraassay CV | 4.4% |
Incubation Time | 4 hr. |
Gene Symbol | DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF |
Total Assay Time | 4 hr. |
Sample Volume | Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL |
Assay Sensitivity | 1.7 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 15.6-1000 pg/mL |
Marker Gene | TNF-α |
For Use With (Application) | Colorimetric Detection |
Test Time | 1 hr. 20 min. |
Accession Number | P01375 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 7124 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ IL-10 Human ELISA Kit
Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.
Measurement | Absorbance at 450nm minus absorbance at 550nm |
---|---|
Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
Target Species | Human |
Conjugate | Biotin |
Description | Human IL-10 ELISA Kit |
Interassay CV | <10% |
Intraassay CV | <10% |
Gene Symbol | CSIF,GVHDS,IL-10A,TGIF,IL10 |
Total Assay Time | 3 hr. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL |
Assay Sensitivity | <3 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 15.36-600 pg/mL |
Test Time | 1 hr. 20 min. |
Accession Number | P22301 |
Regulatory Status | RUO |
Target | Human |
Specificity | No significant cross-reaction with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10 |
Precision | Intra-assay CV <10%; inter-assay CV <10% |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3586 |
Sample Type | Plasma,Serum,Supernatant,Urine |
Label or Dye | HRP |
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 3.7% |
Intraassay CV | 3.5% |
Gene Symbol | CSIF,GVHDS,IL-10A,TGIF,IL10 |
Total Assay Time | 5 hr. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | <1 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 7.8-500 pg/mL |
For Use With (Application) | Colorimetric |
Test Time | 1 hr. 20 min. |
Accession Number | P22301 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | <1pg/mL |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 3586 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
Invitrogen™ ELISA Stop Solution
A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.
Shipping Condition | Wet Ice |
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Content And Storage | Store at 2°C to 8°C |
Form | Liquid |
Product Type | ELISA Stop Solution |
Substrate Type | HRP, TMB |
Invitrogen™ TNF alpha Human ELISA Kit, Ultrasensitive
Quantifies natural and recombinant human Tumor Necrosis Factor Alpha (TNF-α) in serum, plasma, buffered solution, or cell culture medium
Target Species | Human |
---|---|
Conjugate | Biotin |
Interassay CV | 8.5% |
Intraassay CV | 5.9% |
Gene Symbol | DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF |
Total Assay Time | 3 hr. |
Sample Volume | Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL |
Assay Sensitivity | <0.09 pg/mL |
Storage Requirements | 2°C to 8°C |
Assay Range | 0.5-32 pg/mL |
For Use With (Application) | Colorimetric |
Test Time | 1 hr. 20 min. |
Accession Number | P01375 |
Regulatory Status | RUO |
Target | Human |
Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
Precision | <0.09pg/mL |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 7124 |
Sample Type | Plasma,Serum,Supernatant |
Label or Dye | HRP |
BD Human TNF ELISA Kit II
For quantitative determination of human TNF in serum , plasma, and cell culture supernatant
Protein Family | Other Proteins |
---|---|
Target Species | Human |
Conjugate | Biotin |
Interassay CV | <12% |
Intraassay CV | <10% |
Total Assay Time | 4 hr. 45 min. |
Assay Sensitivity | 50 pg/mL |
Storage Requirements | 2°C to 8°C |
Research Discipline | Biomarker-Related Pathways |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hr. 20 min. |
Assay | Solid-phase sandwich ELISA |
Regulatory Status | RUO |
Target | Human |
Specificity | Detect human IgG1. No cross reactivity was found againt IgG2, IgG3, IgG4, IgM, IgA, IgD and IgE |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Label or Dye | HRP |
Protein Family | Cytokines & Receptors |
---|---|
Target Species | Mouse |
Conjugate | Biotin |
Interassay CV | <12% |
Intraassay CV | <10% |
Gene Symbol | A630026I06Rik,Il-7,hlb368 |
Total Assay Time | 4 hr. 45 min. |
Assay Sensitivity | 10 pg/mL |
Storage Requirements | 2°C to 8°C |
Research Discipline | Bone Research |
For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
Test Time | 1 hr. 20 min. |
Assay | Solid-phase sandwich ELISA |
Accession Number | P10168 |
Regulatory Status | RUO |
Target | Mouse |
Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
For Use With (Equipment) | Colorimetric Microplate Reader |
Product Type | ELISA Kit |
Gene ID (Entrez) | 16196 |
Label or Dye | HRP |
Synonym | A630026I06Rik, Il-7, hlb368 |