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Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Flow Rate | 1 to 4 mL/min |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | HiTrap Talon crude prepacked Column |
| Material | Polypropylene |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
| Max. Pressure | 3 bar |
Cytiva Capto™ S Chromatography Media
Designed for capture and intermediate purification of proteins from large feed volumes. Cytiva CaptoTM S Chromatography Media is a strong cation exchange BioProcess medium, designed to meet the demands of modern large-scale manufacturers.
Cytiva Sepharose™ Fast Flow Cation Exchange Media
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
| Particle Size | 45 to 165 μm |
|---|---|
| pH Range | 4 to 13 |
Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media
USe for separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Cytiva Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes.
Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin
Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | Strep-Tactin XT |
| Density (Metric) | Approx. 5 mg/mL resin |
| Matrix | Rigid, cross-linked agarose, spherical |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 6 to 10 pH |
| Binding Capacity | Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin |
| Storage Requirements | 2 to 8°C in 20% ethanol |
| For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag |
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva DEAE Sepharose™ Fast Flow Chromatography Media
For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.
Cytiva TALON™ Superflow Medium
Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | Talon Superflow Media |
| Binding Capacity | Up to 20 mg Histidine-tagged Protein |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography |
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media
Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases
Cytiva MabSelect™ Chromatography Media
Protein A affinity medium designed for capturing MAbs from large sample volumes
| Includes | Certificate of Analysis |
|---|---|
| Particle Size | 85 μm |
| Ligand Type | Recombinant Protein A (E. coli) |
| Flow Rate | <500 cm/Hoμr |
| Matrix | Highly Cross-Linked Agarose |
| pH Range | 3 to 10 |
| Product Type | MabSelect Media |
| Binding Capacity | 30 mg Human lgG/mL (at 2.4 min. residence time) |
| Height (English) Bed | 7.87 in. |
| Storage Requirements | 2°C to 8°C |
| For Use With (Application) | For Industrial AppliCatIons and Well-Established in Approved Processes |
| Height (Metric) Bed | 20 cm |
| Max. Pressure | <200 kPa |
| Particle Size | 75 μm |
|---|---|
| Ligand Type | Recombinant protein (Mr 13 000) |
| Matrix | Highly Cross-linked Agarose |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 3 to 10 |
| Product Type | HiTrap KappaSelect Media |
| Binding Capacity | 15 mg Fab/mL of medium2) |
| Quantity | 5 x 1 mL |
| Material | Polypropylene |
| Storage Requirements | 4 to 8 deg.C, 20% Ethanol |
| Max. Pressure | 300 kPa |