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Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

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Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Invitrogen™ Dynabeads™ Oligo(dT)₂₅

Dynabeads™ Oligo(dT)₂₅ mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information-bearing nucleic acid is mRNA.

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Specifications

RNA Isolation and Purification Products

Shipping Condition	Room Temperature
Ligand Type	Oligo-dT
High-throughput Compatibility	High-throughput Compatible

Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

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Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Standard
Label or Dye	SYBR Green
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	SYBR™
Detection Method	SYBR

Invitrogen™ UltraPure™ DNA Typing Grade™ 50X TAE Buffer

Most commonly used buffer for agarose DNA electrophoresis

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DNA Extraction and Purification

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

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Real Time PCR Assays and Arrays

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

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Specifications

cDNA Synthesis Kits and Master Mixes

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	120 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Concentration	50 U/μL
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

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Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Product Type	High-Fidelity DNA Polymerase
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Standard PCR,High-fidelity PCR

Invitrogen™ Lipofectamine™ 2000 Transfection Reagent

Versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines

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Transfection Reagents

Invitrogen™ TRIzol™ Reagent

TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.

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Specifications

RNA Isolation and Purification Products

Shipping Condition	Room Temperature
Final Product Type	Total RNA, DNA, Protein
Sample Type	Bacteria,Cells,Plants,Tissue,Yeast
Isolation Technology	Organic Extraction
Starting Material Amount	Bacteria: ≤10⁷ cells Cells: ≤10⁷ Plant: ≤10⁷ cells, 100 mg Tissue: ≤100 mg Yeast: ≤10⁷ cells
Purification Time	1 hr.
Yield	DNA: ≤7 μg RNA: ≤73 μg Protein: varies
Elution Volume	20 to 600 μL
For Use With (Application)	RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
High-throughput Compatibility	Not High-throughput Compatible (Manual)

Invitrogen™ RNaseZap™ RNase Decontamination Solution

RNaseZap™ RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap™ Solution onto the surface to be decontaminated and rinse it off with RNase-free water.

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Specifications

Nucleic Acid Controls

Product Type	Surface decontamination solution

Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

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Specifications

dNTPs

Shipping Condition	Dry Ice
Purity or Quality Grade	>99%
Form	Liquid
Label or Dye	Unlabeled
Concentration	10 mM

Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

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Competent Cells

Invitrogen™ RNAlater™ Stabilization Solution

RNAlater RNA Stabilization Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.

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RNA Isolation and Purification Products

Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

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Specifications

Transfection Reagents

Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Product Type	Transfection Reagent
Transfection Technique	Lipid-based Transfection
Sample Type	Plasmid DNA,Synthetic siRNA,RNAi Plasmids (shRNA, miR)
Classification	Animal Origin-Free
Serum Compatible	Yes
Green Features	Sustainable packaging
For Use With (Application)	Transfection
Product Line	Lipofectamine™
Cell Type	Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells

Gibco™ FreeStyle™ 293 Expression Medium

An animal origin-free, serum-free, protein-free formulation, allowing for easier purification

Pricing and Availability
Specifications

Additional Selective and Differential Cell Culture Media

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Product Type	Expression Medium
Form	Liquid
Classification	Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Product Line	Freestyle™
Serum Level	Serum-free

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Invitrogen™ UltraPure™ DNA Typing Grade™ 50X TAE Buffer Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ Lipofectamine™ 2000 Transfection Reagent Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ Lipofectamine™ 3000 Transfection Reagent Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ FreeStyle™ 293 Expression Medium Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Invitrogen™ UltraPure™ DNA Typing Grade™ 50X TAE Buffer

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

Invitrogen™ Lipofectamine™ 2000 Transfection Reagent

Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Gibco™ FreeStyle™ 293 Expression Medium