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Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | SYBR™ |
Detection Method | SYBR |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
Shipping Condition | Dry Ice |
---|---|
Format | Kit |
Reverse Transcriptase | MultiScribe |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 120 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Concentration | 50 U/μL |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Product Type | High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ dNTP Mix (10 mM each)
Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
Shipping Condition | Dry Ice |
---|---|
Purity or Quality Grade | >99% |
Form | Liquid |
Label or Dye | Unlabeled |
Concentration | 10 mM |
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Store at room temperature. |
Form | Liquid |
Packaging Type | Bottle |
Purity | Molecular Biology Grade |
pH | 8 |
Chemical Name or Material | EDTA |
Concentration | 0.5 M |
Product Line | Ambion™ |
Invitrogen™ UltraPure™ BSA (50 mg/mL)
Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.
Shipping Condition | Dry Ice |
---|---|
Content And Storage | Store at –20°C. |
Form | Liquid |
Concentration | 50 mg/mL |
Product Line | Ambion™ |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 30 to 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
For Use With (Application) | RT-PCR |
Format | Kit |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Shipping Condition | Dry Ice |
---|---|
Format | Kit |
Reverse Transcriptase | MultiScribe |
Size (Final Product) | Up to 7 kb |
Reaction Format | Premixed Components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 to 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 37°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Invitrogen™ MgCl2 (1 M)
Molecular biology grade, Invitrogen 1M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ NaCl (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M NaCl solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Store at room temperature. |
Concentration | 5 M |
Product Line | Ambion™ |
Invitrogen™ Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum SuperFi II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >300X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
Shipping Condition | Room Temp or Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Green Features | Sustainable packaging |
Concentration | 2X |
For Use With (Application) | Cloning,Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold 360 DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |