One-Step Reverse Transcription PCR Reagents
One-Step Reverse Transcription PCR Reagents
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Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum Taq DNA Polymerase |
Format | One-Step RT-PCR System |
Size (Final Product) | Up to 4.5 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 50°C |
Technique | 1-Step RT-PCR |
Starting Material | RNA |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Medium |
Content And Storage | Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
Form | Liquid |
Concentration | 4X |
For Use With (Application) | Pathogen Detection,Virus Detection,Real Time PCR (qPCR),Gene Expression |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Detection Method | Primer-probe |
Polymerase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
Format | Single-Tube |
Passive Reference Dye | ROX™ |
Reaction Speed | Fast or Standard |
Hot Start | Antibody-based |
Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
Product Line | TaqPath™ |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum Taq High Fidelity |
Format | One-Step RT-PCR System |
Size (Final Product) | Up to 10 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 50°C |
Technique | 1-Step RT-PCR |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum SuperFi |
Format | Kit |
Size (Final Product) | Up to 14 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 45°C to 60°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
GC-Rich PCR Performance | High |
---|---|
Format | 1-step RT-PCR Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 13 kb |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Hot Start | Built-in Hot Start |
Optimal Reaction Temperature | 50°C to 65°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
PCR Method | 1-step RT-PCR |
Multiplex Capability | Yes |
Detection Method | Gel Electrophoresis |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
GC-Rich PCR Performance | Medium |
Content And Storage | Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
Format | Single-Tube |
Form | Liquid |
Reaction Speed | Fast or Standard |
Hot Start | Antibody-based |
Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
For Use With (Application) | Virus Detection,Pathogen Detection,Real Time PCR (qPCR),Gene Expression |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Thermo Scientific™ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq
Thermo Scientific Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq is a complete set of RT-PCR reagents that combines everything needed for RT-PCR.