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Enzymes and Inhibitors

Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
Restriction Enzymes (360)
Modifying Enzymes (3)

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1 – 15 of 325 results
1

Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

Promotions are available
Cut Site CTCTTC(1/4)^
Content And Storage Store at -20°C.
Form Liquid
Enzyme Eam1104I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity 150 μL
Type IIS RE Yes
Incubator Temperature 37°C
Concentration 20 U/μL
For Use With (Application) In vitro Transcription
Inactivation Temperature 65°C
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Research Category Drug Development
Compatible Buffer 10X Tango Buffer
Optimal Reaction Temperature 37°C
Product Line TheraPure™
2

Thermo Scientific™ Buffer Set for Restriction Enzymes

Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.

3

Thermo Scientific™ Dilution Buffer for Restriction Enzymes

Optimize applications that require diluted enzymes with this dilution buffer.

4

Thermo Scientific™ FastDigest NdeI

The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Nde I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
5

Thermo Scientific™ FastDigest BpiI (IIs class)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).

Promotions are available
6

Thermo Scientific™ FastDigest NheI

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Nhe I
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
7

Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

Promotions are available
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Nco I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
8

Thermo Scientific™ FastDigest SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme SacI
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 3' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
9

Thermo Scientific™ FastDigest Eco31I (IIs class)

The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)

Promotions are available
EDGE
Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Eco31I
Methylation Sensitivity CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive
Research Category Scarless Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE Yes
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest™
10

Thermo Scientific™ FastDigest Esp3I (IIs class)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Promotions are available
11

Thermo Scientific™ FastDigest BcuI

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).

Promotions are available
EDGE
Sensitive to Heat Inactivation No
Product Type Restriction Enzyme
Enzyme Bcu I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang 5' protruding ends
Type IIS RE No
Optimal Reaction Temperature 37°C
Product Line FastDigest™
12

Thermo Scientific™ FastDigest PvuII

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Promotions are available
13

Thermo Scientific™ FastDigest BshTI

Cut at A^CCGGT sites with Thermo Scientific™ FastDigest™ AgeI (BshTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Promotions are available
EDGE
14

Thermo Scientific™ FastDigest Eco32I

Cut at GAT^ATC sites with Thermo Scientific™ FastDigest™ EcoRV (Eco32I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: EcoRV).

Promotions are available
Sensitive to Heat Inactivation No
Product Type Restriction Enzyme
Enzyme Eco32I
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang Blunt
Type IIS RE No
Optimal Reaction Temperature 37°C
Product Line FastDigest™
15

Thermo Scientific™ NcoI (10 U/μL)

The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).

Promotions are available
Concentration 10 U/μL