IBA Lifesciences

IBA Lifesciences provides high performance research tools for cell and protein isolation based on their proprietary Strep-tag™ technology. Their product portfolio offers solutions for the entire production chain of recombinant proteins, from cloning, transfection, protein expression and purification to detection, immobilization and assays, as well as innovative cell isolation and expansion tools.

IBA's Cell Selection and Expansion

TACS - Traceless Affinity Cell Selection

Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection:

High purity, yield and viability
Label-free, non-activated cells due to fully reversible binding
From whole blood, buffy coat, PBMCs or murine samples
Affinity chromatographic, magnetic or fluorescent cell separation

TACS - Traceless Affinity Cell Selection

MHC I Streptamer™

Detection and isolation of antigen-specific T-cells with MHC I Streptamers™:

Label-free cell isolation according to antigen specificity
High specificity, sensitivity, and reproducibility
From single cell suspensions (e.g., PBMCs or murine samples)
Affinity chromatographic, magnetic or fluorescent cell separation

Cell Stimulation and Expansion

CD3: CD28 Streptamers™:

Completely reversible reagents
Termination of stimulation at any given point in time
Adjustable CD3: CD28 ratio
Bead-free

Peptides for T-Cell Stimulation:

Purity >85%
Endotoxin-free

Fluorescent Cell Staining

Detection of antigen-specific T-cells with MHC I Streptamers™ and of receptor-specific T-cells with Fab Streptamers™:

Fully reversible
High specificity, sensitivity, and reproducibility
No leftover staining reagents on the cells after FACS sorting
Optimal preservation of cell effector function
Improved viability of cells

Protein Production and Assay

Cloning

More than 150 Twin-Strep-tag™/Strep-tag™II expression vectors:

Variety of Twin-Strep-tag™/Strep-tag™ II combined with His, FLAG or GST
Different N-/C-terminal orientations
Expression hosts: E. coli, mammalia, insect cells, yeast
Easy cloning via StarGate vectors

Mammalian Expression

The MEXi mammalian expression system is optimised for efficient protein expression and Strep-tag™ purification in mammalian cells:

Perfectly matched HEK293E cell line and cell culture media
Transient expression for time and cost efficient protein production
Multiple expression vectors with different features

Protein Purification

Strep-tag™ technology from the original manufacturer:

High affinity in low pM range
Rapid one-step purification with unparalleled protein purity (>95%)
Mild and specific elution
Tolerates broad range of buffer conditions
Suitable for metalloproteins, membrane proteins, low abundant proteins, and protein complexes amongst others

Detection

For Western blot analysis, immunofluorescence, FACS and ELISA.

Two different detection systems are provided:

Strep-Tactin™ and Strep-Tactin™ XT conjugate for Twin-Strep-tag™/Strep-tag™ II proteins
Monoclonal antibodies against Twin-Strep-tag™/Strep-tag™ II

Immobilization

With near covalent binding affinity, – Immobilisation by Strep-Tactin XT: Twin-Strep-tag can be used for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, surface plasmon resonance (SPR) experiments (Biacore) or bio-layer interferometry (BLI):

High affinity in low pM range enables measurements over a long time
Minimal non-specific binding
Minimal coefficients of variation (cv)
Chip for SPR experiments can be easily regenerated

Surface-Marker Specific Exosome Isolation

Quick and simple isolation of unlabeled exosomes using Fab-TACS™ for exosomes, which offers:

Highly specific and reproducible isolation
Minimal contamination by other EVs
Label-free exosomes with intact biological functions
Source-independent application
Reversible capture and release of exosomes
A quick and simple protocol, without magnetic beads or ultracentrifugation

Surface-marker Specific Exosome Isolation

Immobilization with Near Covalent Binding Affinity

Reversible capture and analysis of Twin-Strep-tag™ fusion proteins for bio-layer interferometry (BLI) or surface plasmon resonance (SPR) experiments:

Indirect immobilization of the ligand prevents it from being subject to inactivation or influences of the immobilization procedure
High specificity and affinity in low pM range of the Twin-Strep-tag™ - Strep-Tactin™ XT interaction
Ligands can be captured directly from complex samples
Strep-Tactin™ XT biosensors/chips can be easily regenerated by an established procedure, whereby further ligand-analyte interactions can be analyzed with the same biosensor/chip

Immobilization with Near Covalent Binding Affinity

Our Perfect Tool for Isolation of Proteins or Complexes

MagStrep 'Type 3' XT Magnetic Beads offer:

High specificity and low background resulting in high protein purity
High binding affinity and capacity for Strep-tag™ II or Twin-Strep-tag™ fusion proteins
Immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) in batch format and/or high throughput screening for protein purification via pull-downs
Suitable for usage with (semi-)automated magnetic bead purification systems (e.g., Thermo Scientific™ KingFisher™) for automated workflows

Our Perfect Tool for Isolation of Proteins or Complexes

Efficient Purification Even of Large Proteins

The Strep-Tactin™ XT 4Flow™ Starter Kit consists of everything you need for a quick start:

Strep-Tactin™ XT 4Flow™ gravity flow column
All buffers required for washing, elution, and regeneration
Strep-Tactin™ conjugated with horseradish peroxidase (HRP; for Western blot detection)
Short protocol

Efficient Purification Even of Large Proteins

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

Applicable for purification, detection, and immobilization of biotinylated molecules:

Suitable for proximity-dependent biotin identification (BioID) experiments
Mild and highly specific elution facilitating analysis of protein complexes
Strep-Tactin™ Sepharose™ and Strep-Tactin™ Superflow™ for gravity flow column-based affinity purification
MagStrep 'Type 3' Strep-Tactin™ magnetic beads are suitable for batch purification as well as pull-down assays

Strep-Tactin™ for Reversible Binding of Biotinylated Proteins

Cell Selection and Expansion

Filename: ApplicationNote-Central-memory-T-cells-isolated-and-stained-with-CD8α-and-CD62L-Fab-Streptamer-reagents.pdf
Size: 662 KB
Format: application/pdf

Application Note Combining MHC-I and CD8+ Fab Streptamer reagents

Filename: ApplicationNote-Combining-MHC-I-and-CD8+-Fab-Streptamer-reagents.pdf
Size: 803 KB
Format: application/pdf

Filename: ApplicationNote-Regulatory-T-cells-isolated-and-stained-with-CD4-CD25-and-CD45RA-Fab-Streptamer-reagents.pdf
Size: 737 KB
Format: application/pdf

Filename: ApplicationNote-CD3-T-Lymphocytes-isolated-and-stained-with-Streptamer-reagents.pdf
Size: 200 KB
Format: application/pdf

Filename: Application_Note_Negative_cell_selection_using_affinity_chromatography.pdf
Size: 1 MB
Format: application/pdf

Filename: Application_Note_Depletion_of_cell_populations_from_different_samples.pdf
Size: 1 MB
Format: application/pdf

Filename: White_Paper_Understanding_The_World_of_MHC_I_Multimers.pdf
Size: 5 MB
Format: application/pdf

Protein Production and Assay

ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-High-Capacity

Filename: ApplicationNote-Strep-Tactin-XT-vs-Strep-Tactin-high-capacity.pdf
Size: 193 KB
Format: application/pdf

Filename: ApplicationNote-Strep-Tactin-high-capacity.pdf
Size: 100 KB
Format: application/pdf

Filename: ApplicationNote-Strep-Tactin-XT-Assay.pdf
Size: 261 KB
Format: application/pdf

IBA-Application Note_STvsXT.indd

Filename: ApplicationNote-Strep-Tactin-vs-Strep-Tactin-XT.pdf
Size: 917 KB
Format: application/pdf

Filename: ApplicationNote-Twin-Strep-tag-vs-His-tag.pdf
Size: 675 KB
Format: application/pdf

BLI sSPR8 - Kopie.indd

Filename: Application_note_BLISPR_with_Strep-TactinXT.pdf
Size: 1 MB
Format: application/pdf

Filename: White_paper_Comparison_protein_purification_systems.pdf
Size: 1 MB
Format: application/pdf

20191111_ResearchGate_Expi.indd

Filename: White_paper_Transient_expression_in_mammalian_cells.pdf
Size: 1 MB
Format: application/pdf

Exosomes

Application_note_exosomes_MSC.indd

Filename: Application_note_exosome_isolation_from_MSC.pdf
Size: 1 MB
Format: application/pdf

Application_note_exosomes_Jurkat_Serum.indd

Filename: Application_note_exosome_isolation_from_Jurkat_Serum.pdf
Size: 782 KB
Format: application/pdf

IBA Lifesciences

IBA's Cell Selection and Expansion

TACS - Traceless Affinity Cell Selection

MHC I Streptamer™

Cell Stimulation and Expansion

Fluorescent Cell Staining

Cell Biology Promotion

Chemical Extravaganza

Cleanroom Essentials Extravaganza

Contract Testing Lab

Dairy and Plant-Based Products Testing Promotion

Essential Lab Wipes and Paper Sale

Exclusive Discounts on Storage Solutions: Storage Tubes, Equipment and Accessories

Exclusive Promotion on Equipment and Instruments

Exclusive Promotion on Liquid Handling

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